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Sins of the mother shall not be visited upon the children




Fetal programming is the process by which early environment interacts with genes to produce an individual human constitution. It is not only of great interest to developmental scientists but also has high media resonance due to the more and more commonly acknowledged notion that pregnancy actually affects vulnerability to disease later in life. First evidence came from an epidemiological study showing that birth characteristics, like low weight and lower gestational age, were linked to later diseases like diabetes, hypertension, but also psychopathology. What is currently accepted, though, is that these individual characteristics at birth are not the actual risk factors for later disease but rather is the environment to which the mother and the fetus are exposed during pregnancy that affects fetal development itself and in turn development later in life. There comes the focus of this post on actual prenatal conditions that may increase the risk for disease later in life, with a specific focus on mental health.

A key assumption is that biological systems undergoing rapid developmental changes are especially vulnerable to organizing and disorganizing influences. Once we accept this key assumption, it becomes logic to consider the brain a key vulnerable system to environmental influences, with 100 billion neurons and 100 trillion connections resulting from a 3mm long neural tube just after conception.


Early environment interacts with genes to eventually increase vulnerability to disease later in life. In simple terms, genes do frame development but it is early environment that actually sculpts it. It all starts before birth, during pregnancy, when conditions like maternal stress, obesity, infection, inflammation, and drug exposure, including alcohol use, might impact later psychopathology. A way to see the effects of early environment is through the analysis of brain alterations in newborns followed up longitudinally across several years over development. Here, I will focus on stress and its effect on the fetus through the mother as I think it is, together with anxiety, the disease of the century. Many of us have experienced or currently suffer from anxiety, and I think nobody can say not to be stressed at all nowadays. If it is the disease of today, how much do we know about it? How can we handle it? And can we make sure it does not become the disease of tomorrow? I will not be able to answer all these questions here, but will try to convey the message that it can be the disease of tomorrow with all likelihood, and we are making sure it passes on.

Maternal stress during pregnancy is a risk factor for children’s cognitive impairment and behavioural issues in toddlerhood. Higher anxiety in mothers during pregnancy was linked to lower brain matter volume in brain areas associated with functions like language, memory and attention, and was associated to the correspondent cognitive impairments in 7-year-olds. Similarly, prenatal maternal depression was linked to child cortical thinning in frontal areas at 7 years of age, mediating the association with externalizing symptoms in those children. These brain alterations affect behaviour in different ways, both directly in the associated function, but also indirectly by changing the way the child learns from and adapts to the environment over development. Looking at newborns, researchers found an association of maternal stress with small hippocampal volume, a key region for memory. This brain alteration can affect how the child responds and adapts to a richer and more stimulating environment over development, resulting in different cognitive levels and behavioural profiles. Specifically, newborns with larger hippocampus could benefit more from a stimulating environment in relation to later cognitive function, suggesting differential susceptibility to environmental influences over development based on prenatal environment.

Many brain imaging studies have been conducted to investigate the association between maternal stress, depression and anxiety and brain development in the child. These studies identified various alterations in both anatomy and connectivity of the brain in fetuses, children, as well as adolescents. But how does the fetus know that mom is stressed? and how does the fetus react? First, stress is very much personal at a behavioural level, as can be related to work, family issues, or anything else. But for the fetus to know that mom is stressed, stress has to be biologically based. There is no neural connection between mom and fetus and all happens through placenta, so here comes cortisol, which is produced during stress responses and can pass through placenta to the fetus. Cortisol is fundamental for brain development in terms of cellular growth and functioning and so important for the fetus to receive, just not too much. A specific enzyme is in charge to convert cortisol from active to inactive in placenta when it passes through, although 10 to 20% still remains active. If mom is stressed, though, her own level of cortisol increases plus that specific enzyme is less effective, leading to an increase in cortisol concentration in the fetus. This can lead to alterations in brain development affecting neuronal proliferation and differentiation, and has been linked to neurodevelopmental disorders like autism. Higher cortisol at 15 weeks of gestation was associated to higher amygdala volume and amygdala connectivity to somatosensory and frontal areas in baby girls, and such larger amygdala mediated affective symptoms, in particular the increase in internalizing behaviours at 3 years of age. It is not clear why females are more affected here, and in fetal programming in general, but potentially supports the idea of adaptation to higher vigilance in an environment considered to be hostile during development.


Thus, early life stress caused by conditions like maternal stress during pregnancy acts on alterations of the endocrine system (cortisol), which acts as mediator for altered brain development eventually leading to altered cognitive and affective processes and overall mental health issues. Of note, cortisol is needed for fetal development anyway. It is not a toxic substance or so, but it’s just the concentration levels to be altered in response to adverse environment during pregnancy. From an evolutionary point of view, the resulting behavioural characteristics can be also thought as adaptation to the early disruptive environment. An example? Anxiety can be seen as increased vigilance which can be protective in a dangerous environment. Thus, we should keep in mind that all this is the result of adaptive mechanisms to change in concentrations of biological substances that are supposed to be there to prepare the fetus to the external world.

Other gestational conditions, including obesity and prenatal inflammation, have been shown to be risk factors for various neurodevelopmental disorders, including autism and schizophrenia. A key substance that increases in concentration levels in response to stress or depression is interleukin 6, involved in inflammatory processes. In stressed moms, it can pass through placenta directly or stimulate placental cytokine production resulting in release of inflammatory cytokines like interleukine 6 in the fetal compartment, where they reach the fetus brain. Interleukin-6 has also been associated to higher amygdala volume and more connectivity to brain areas involved in learning and memory in baby boys as well as girls. These brain alterations during the first year of life can eventually lead to alterations in cognition and general functioning, as indicated by cognitive impairments at age 1 lower intelligence at age 4. Thus, immune activation and inflammation of moms during pregnancy affect their own children’s behaviour in toddlerhood by acting on connectivity between their salience and attentional brain networks as newborns. Researchers have also found that antibodies to this interleukin-6 can block the effects of maternal inflammation on offspring stress reactivity, social deficits and learning impairments.

Finally, it is important to include in this framework some considerations on mom’s past experiences that might affect her behaviour during pregnancy and beyond that. It is, in fact, possible that not only what mom experiences during pregnancy affects the child’s development but also what she experienced before, as a child herself, like childhood trauma. We know that childhood trauma is a risk factor for psychiatric disorders as well as or other biological alterations like obesity, altered response to stress and higher levels of inflammation. A mom brings along her own physiological alterations during pregnancy, which in turn affect the child’s development. The sad cycle of childhood trauma can be set in motion by changing the parenting behaviour of a mom or dad (see neglect or abuse), leading to further trauma in the child that may result in developmental conditions like autism. Even if the cycle is broken and the child is not directly exposed to trauma, the package of childhood trauma that mom brings along can have an intergenerational effect on the child, for instance through physiological alterations (like stress response, inflammation and obesity) or behavioural alterations (like drug and alcohol abuse, depression, anxiety and altered parenting style in general).



To conclude, I showed you the tip of the iceberg of something as fascinating and complex as fetal programming. I focused on the stress-related part of this topic due to the high pertinence in our lives as we are living in such a frenetic society. We are all stressed out, with a long list of consequences for ourselves and our offspring. But how could we not in crazy times like these when we just needed a pandemic to make things even worse? This is not something easy to solve, but it would be useful to acknowledge the issue, raise awareness and try to do what we can to improve things a bit, like a change of view on current lifestyle and priorities.

Rasmussen LJH, Moffitt TE, Arseneault L, et al. Association of Adverse Experiences and Exposure to Violence in Childhood and Adolescence With Inflammatory Burden in Young People [published online ahead of print, 2019 Nov 4]. JAMA Pediatr. 2019;174(1):1-11. doi:10.1001/jamapediatrics.2019.3875

Conradt E, Adkins DE, Crowell SE, Raby KL, Diamond LM, Ellis B. Incorporating epigenetic mechanisms to advance fetal programming theories. Dev Psychopathol. 2018;30(3):807-824. doi:10.1017/S0954579418000469

Graham AM, Rasmussen JM, Entringer S, et al. Maternal Cortisol Concentrations During Pregnancy and Sex-Specific Associations With Neonatal Amygdala Connectivity and Emerging Internalizing Behaviors. Biol Psychiatry. 2019;85(2):172-181. doi:10.1016/j.biopsych.2018.06.023

Entringer S, Buss C, Wadhwa PD. Prenatal stress, development, health and disease risk: A psychobiological perspective-2015 Curt Richter Award Paper. Psychoneuroendocrinology. 2015;62:366-375. doi:10.1016/j.psyneuen.2015.08.019

Sandman CA, Davis EP, Buss C, Glynn LM. Exposure to prenatal psychobiological stress exerts programming influences on the mother and her fetus. Neuroendocrinology. 2012;95(1):7-21. doi:10.1159/000327017

Buss C, Entringer S, Swanson JM, Wadhwa PD. The Role of Stress in Brain Development: The Gestational Environment's Long-Term Effects on the Brain. Cerebrum. 2012;2012:4.

Buss C, Davis EP, Hobel CJ, Sandman CA. Maternal pregnancy-specific anxiety is associated with child executive function at 6-9 years age. Stress. 2011;14(6):665-676. doi:10.3109/10253890.2011.623250

Buss C, Davis EP, Muftuler LT, Head K, Sandman CA. High pregnancy anxiety during mid-gestation is associated with decreased gray matter density in 6-9-year-old children. Psychoneuroendocrinology. 2010;35(1):141-153. doi:10.1016/j.psyneuen.2009.07.010



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